Journal: Journal of Hepatocellular Carcinoma

Article Title: Coilin Affects the Prognosis of Hepatocellular Carcinoma Through Cell Cycle and Apoptosis

doi: 10.2147/JHC.S500119

Figure Lengend Snippet: Expression of COIL in HCC cell lines and HCC tissue from hospital. The expression of COIL in liver cancer cell lines huh7, hepG2 and hep3B was higher than that in L02 by immunohistochemistry ( A ) and Western blotting ( B ). ( C ) The relative expression of COIL in HCC was significantly higher than that in normal liver tissue by qRT-PCR (***p value < 0.001). ( D ) The expression of COIL in HCC tissue was higher than that in normal liver tissue. ( E, F ) Representative immunohistochemistry results of low or high COIL expression. ( G ) High COIL expression was associated with poor overall survival.

Article Snippet: The human normal liver cell line L02 was obtained from Beijing Baiao Bowei Biotechnology Co., Ltd.

Techniques: Expressing, Immunohistochemistry, Western Blot, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling

doi: 10.1038/srep35131

Figure Lengend Snippet: Changes in the geometrical parameters of HCCs and NHs before and after CII.

Article Snippet: Five HCC lines, HepG2, Smmc-7721, Huh-7, Mhcc-97L, and Mhcc-97H, and a control NH line, L02, were obtained from the China Center for Type Culture Collection (Shanghai, China).

Techniques: Cell Analysis, Single-cell Analysis

Journal: Scientific Reports

Article Title: Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling

doi: 10.1038/srep35131

Figure Lengend Snippet: ( A ) Cell shape vs mechanical properties. In detail, CPA vs E, in L02, r = 0.642, P = 0.00 < 0.01; in HepG2, r = 0.619, P = 0.00 < 0.01; in Smmc-7721, r = 0.519, P = 0.00 < 0.01; in Huh-7, r = 0.564, P = 0.00 < 0.01; in Mhcc-97L, r = 0.355, P = 0.005 < 0.01; ( B ) Cytoskeleton density vs mechanical properties. In detail, CD vs E, in L02, r = 0.872, P = 0.00 < 0.01; in HepG2, r = 0.790, P = 0.00 < 0.01; in Smmc-7721, r = 0.876, P = 0.00 < 0.01; in Huh-7, r = 0.866, P = 0.00 < 0.01; in Mhcc-97L, r = 0.724, P = 0.00 < 0.01; (C) Cell shape vs cytoskeleton density. In detail, CPA vs CD, in L02, r = 0.841, P = 0.00 < 0.01; in HepG2, r = 0.784, P = 0.00 < 0.01; in Smmc-7721, r = 0.700, P = 0.00 < 0.01; in Huh-7, r = 0.568, P = 0.00 < 0.01; in Mhcc-97L, r = 0.501, P = 0.003 < 0.01; ( D ) Caspase-3 protein levels vs mechanical properties. In detail, CPLs vs E, in L02, r = −0.811, P = 0.00 < 0.01; in HepG2, r = −0.784, P = 0.00 < 0.01; in Smmc-7721, r = −0.714, P = 0.00 < 0.01; in Huh-7, r = −0.747, P = 0.00 < 0.01; in Mhcc-97L, r = −0.701, P = 0.003 < 0.01.

Article Snippet: Five HCC lines, HepG2, Smmc-7721, Huh-7, Mhcc-97L, and Mhcc-97H, and a control NH line, L02, were obtained from the China Center for Type Culture Collection (Shanghai, China).

Techniques:

Journal: Cell Death & Disease

Article Title: Polyphyllin I suppresses the formation of vasculogenic mimicry via Twist1/VE-cadherin pathway

doi: 10.1038/s41419-018-0902-5

Figure Lengend Snippet: a Polyphyllin I exhibited the most obvious inhibitory effect on VM formation. b Structure of PPI. c Effects of PPI on the viability of SMMC7721, PLC, HepG2, Hep3B, and Bel7402 cells. PPI reduced the survival rate of HCC cells at relatively high concentrations and had slight influence on cell survival below 1 μM. d Cell cycle analysis of PPI in HCC cell lines. 0.1 μM PPI did not influence the cycle of HCC cells. e , f PPI inhibited the migration and invasion of HCC cells. g PPI decreased the number of pipe-like structures in SMMC7721 cells on Matrigel. h Time-lapse images of SMMC7721. PPI inhibited the pipe-like structure formation in SMMC7721 cells ( n = 3)

Article Snippet: HCC cell lines including SMMC7721, PLC, HepG2, Hep3B, and Bel7402 were purchased from Keygene BioTECH (Nanjing, China) and validated through a short tandem repeat-based method.

Techniques: Cell Cycle Assay, Migration